Year: 2014 | Month: July | Volume 7 | Special Issue
Molecular study of Pigeonpea [Cajanuscajan (L.) Mill sp.]Genotypes for Fusariumwilt using RAPD markers
Abstract:
The molecular study of seventeen genotypes of pigeonpea using 20 random amplified polymorphic DNA (RAPD) markers has been reported. A total of 179 loci were scored corresponding to an average of 8.95 loci per primer with 123 bands showing polymorphism (65.42%).Very low level of polymorphism in cultivated pigeonpeagermplasm had been earlier reported which was corroborated by many pigeonpea workers indicating the normal genetic base existing in this crop (Odenyet al. 2007). The average number of polymorphic loci obtained per primer (Assay Efficiency Index) was found to be 6.15. Jaccard’s similarity coefficient ranged from 0.52 to 0.77 and the marker index value for pooled RAPD was found to be 11.65. A dendrogram constructed based on the UPGMA clustering method revealed two major clusters. Cluster-I comprised of 5 cultivars whichwas further differentiated into two sub-clusters. The cluster-II included remaining twelve cultivars. Genotypes that are susceptible to fusariumwilt of pigeonpeaviz., GT-1, GT-100, GT-101, GT-102 and BANAS were closely related and they formed one cluster. It also revealed that genotypes viz., AGT-2 and AVPP-1 were closely related and formed one cluster whereas viz., T-15-15, LRG-41, C-11, BDN-2 and ICPL-87 were closely related and formed another cluster. The dendogram showed that genotypes that are resistant to fusarium wilt of pigeonpeaviz., BSMR-853, WRGE-119, ICPL-87119, ICPL-84060 and ICP-8863 were related genotypes and they formed another cluster. The study reiterated that RAPD can be efficiently used for discriminating resistant and susceptible pigeonpea genotypes.
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